A gene cluster encoding malonyl-CoA decarboxylase (MatA), malonyl-CoA synthetase (MatB) and a putative dicarboxylate carrier protein (MatC) in Rhizobium trifolii . Cloning, sequencing, and expression of the enzymes in Escherichia coli
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منابع مشابه
Crystallization and preliminary X-ray crystallographic analysis of malonyl-CoA decarboxylase from Rhizobium leguminosarum bv. trifolii.
Malonyl-CoA decarboxylase (MCD), which catalyzes the conversion of malonyl-CoA to acetyl-CoA, is an evolutionarily distinct and highly conserved enzyme. MCD does not share sequence homology with other known decarboxylases, while the enzymes from different species exhibit at least >30% sequence identity to each other. In order to provide a canonical structure of the enzyme for detailed study of ...
متن کاملRat malonyl-CoA decarboxylase; cloning, expression in E. coli and its biochemical characterization.
Malonyl-CoA decarboxylase (E.C.4.1.1.9) catalyzes the conversion of malonyl-CoA to acetyl-CoA. Although the metabolic role of this enzyme has not been fully defined, it has been reported that its deficiency is associated with mild mental retardation, seizures, hypotonia, cadiomyopathy, developmental delay, vomiting, hypoglycemia, metabolic acidosis, and malonic aciduria. Here, we isolated a cDN...
متن کاملurtica dioica effect on malonyl-coa decarboxylase
background the malonyl-coa decarboxylase (mcd, ec.4.1.1.9) enzyme regulates malonyl-coa levels. the effect of aerial parts extracts of urtica dioica (ud) on mcd is poorly understood. objectives the present experiment was undertaken to evaluate the effect of ud aerial parts extracts on mcd level. materials and methods in this experimental study, two groups of rats were used: normal and hyperglyc...
متن کاملCloning and expression of rat pancreatic β-cell malonyl-CoA decarboxylase
Nicolas VOILLEY*, Raphae$ l RODUIT*, Raffaela VICARETTI*, Christophe BONNY†, Ge! rard WAEBER†, Jason R. B. DYCK‡, Gary D. LOPASCHUK‡ and Marc PRENTKI* *Molecular Nutrition Unit, Department of Nutrition, University of Montreal and the CR Centre Hospitalier de l’Universite! de Montre! al and Institut du Cancer, 1560 Sherbrooke Est, Montreal, Quebec H2L 4M1, Canada, †Department of Internal Medicin...
متن کاملEffects of Teucrium polium aerial parts extracts on malonyl-CoA decarboxylase level
Malonyl-CoA decarboxylase (MCD) is an enzyme involved in the decarboxylation of malonyl-CoA to acetyl-CoA. In order to explore the hypothesis that the changing plant materials’ MCD activity level can serve as therapy to diabetics, the effect of Teucrium polium compounds was studied in a diabetic rat model. In this experimental study, two groups of rats, a control and a diabetic group, each incl...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1998
ISSN: 0014-2956,1432-1033
DOI: 10.1046/j.1432-1327.1998.2570395.x